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poly  (MedChemExpress)


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    Structured Review

    MedChemExpress poly
    Poly, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/poly+i+c/Poly+(I%3AC)%3AKanamycin/pmc13392861-37-0-4
    Average 94 stars, based on 2 article reviews
    poly - by Bioz Stars, 2026-10
    94/100 stars

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    In Vitro:

    Article Title: Epigenetic programming by H3K23ac defines lineage fate of Meg3 + haematopoietic stem cells and drives immune ageing
    Article Snippet: .. For in vitro stimulation, HSCs were treated with 100 ng ml −1 LPS (Sigma) or 100 ng ml −1 Poly(I:C) (MCE) for the specified durations. ..

    Phospho-proteomics:

    Article Title: NMI Regulates Adipose Adaptive Thermogenesis Through TLR4/IRF3 Signaling to Promote Obesity
    Article Snippet: .. To investigate the roles of PPARα, PPARγ, IRF3 signaling, differentiated mature adipocytes were treated with GW6471 (a PPARα antagonist; Selleck, Cat#S2798, 10 μ m ), GW9662 (a PPARγ antagonist; Selleck, Cat#S2915, 10 μ m ), poly(I:C) (an IRF3 phosphorylation inducer; MedChemExpress, Cat#HY‐107202, 50 mg mL −1 ), or amlexanox (an IRF3 inhibitor; MedChemExpress, Cat#HY‐B0713, 50 μ m ) for 24–48 h (GW6471 and GW9662) or 1 h (poly(I:C) and amlexanox), after which the cells were harvested for RNA and protein analysis. ..

    Transfection:

    Article Title: Duck plague virus US2 promotes p62-mediated autophagic degradation of RIG-I to suppress antiviral signaling
    Article Snippet: DEF cells were co-transfected with the IFN-β promoter-driven firefly luciferase reporter plasmid and the Renilla luciferase control plasmid using Hieff Trans® Liposomal 2000 Transfection Reagent (Yeasen Biotechnology, Shanghai, China; Cat# 40802ES03). .. Cells were stimulated with poly(I:C) (MCE, Shanghai, China; Cat# HY-107202) or poly(dA:dT) (Sigma-Aldrich, St. Louis, MO, USA; Cat# P9764) and simultaneously transfected with either the empty vector or the US2 expression plasmid. .. At 36 h post-transfection, luciferase activities were measured using the TransDetect® Dual-Luc Pro Luciferase Reporter Assay Kit (TransGen Biotech, Beijing, China; Cat# FR203-01) with a GloMax® Navigator Microplate Luminometer (Promega, USA).

    Plasmid Preparation:

    Article Title: Duck plague virus US2 promotes p62-mediated autophagic degradation of RIG-I to suppress antiviral signaling
    Article Snippet: DEF cells were co-transfected with the IFN-β promoter-driven firefly luciferase reporter plasmid and the Renilla luciferase control plasmid using Hieff Trans® Liposomal 2000 Transfection Reagent (Yeasen Biotechnology, Shanghai, China; Cat# 40802ES03). .. Cells were stimulated with poly(I:C) (MCE, Shanghai, China; Cat# HY-107202) or poly(dA:dT) (Sigma-Aldrich, St. Louis, MO, USA; Cat# P9764) and simultaneously transfected with either the empty vector or the US2 expression plasmid. .. At 36 h post-transfection, luciferase activities were measured using the TransDetect® Dual-Luc Pro Luciferase Reporter Assay Kit (TransGen Biotech, Beijing, China; Cat# FR203-01) with a GloMax® Navigator Microplate Luminometer (Promega, USA).

    Expressing:

    Article Title: Duck plague virus US2 promotes p62-mediated autophagic degradation of RIG-I to suppress antiviral signaling
    Article Snippet: DEF cells were co-transfected with the IFN-β promoter-driven firefly luciferase reporter plasmid and the Renilla luciferase control plasmid using Hieff Trans® Liposomal 2000 Transfection Reagent (Yeasen Biotechnology, Shanghai, China; Cat# 40802ES03). .. Cells were stimulated with poly(I:C) (MCE, Shanghai, China; Cat# HY-107202) or poly(dA:dT) (Sigma-Aldrich, St. Louis, MO, USA; Cat# P9764) and simultaneously transfected with either the empty vector or the US2 expression plasmid. .. At 36 h post-transfection, luciferase activities were measured using the TransDetect® Dual-Luc Pro Luciferase Reporter Assay Kit (TransGen Biotech, Beijing, China; Cat# FR203-01) with a GloMax® Navigator Microplate Luminometer (Promega, USA).



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    pUS2 suppresses IFN-β production in DEF cells. A. DEF cells were co-transfected with the IFN-β luciferase reporter plasmid, Renilla luciferase control plasmid, and either pUS2 expression plasmid or empty vector. Cells were stimulated with <t>poly(I:C),</t> and IFN-β promoter activity was measured using a dual-luciferase reporter assay. pUL45, a DPV viral protein, was used as a negative control. B. DEF cells were co-transfected as described above and stimulated with poly(dA:dT). IFN-β promoter activity was measured. C. The mRNA expression levels of IFN-β, OASL, and Mx in DEF cells following poly(I:C) stimulation in the presence or absence of pUS2 were determined by RT-qPCR. D. Expression of pUS2 protein was confirmed by Western blotting analysis.
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    pUS2 suppresses IFN-β production in DEF cells. A. DEF cells were co-transfected with the IFN-β luciferase reporter plasmid, Renilla luciferase control plasmid, and either pUS2 expression plasmid or empty vector. Cells were stimulated with <t>poly(I:C),</t> and IFN-β promoter activity was measured using a dual-luciferase reporter assay. pUL45, a DPV viral protein, was used as a negative control. B. DEF cells were co-transfected as described above and stimulated with poly(dA:dT). IFN-β promoter activity was measured. C. The mRNA expression levels of IFN-β, OASL, and Mx in DEF cells following poly(I:C) stimulation in the presence or absence of pUS2 were determined by RT-qPCR. D. Expression of pUS2 protein was confirmed by Western blotting analysis.
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    Image Search Results


    pUS2 suppresses IFN-β production in DEF cells. A. DEF cells were co-transfected with the IFN-β luciferase reporter plasmid, Renilla luciferase control plasmid, and either pUS2 expression plasmid or empty vector. Cells were stimulated with poly(I:C), and IFN-β promoter activity was measured using a dual-luciferase reporter assay. pUL45, a DPV viral protein, was used as a negative control. B. DEF cells were co-transfected as described above and stimulated with poly(dA:dT). IFN-β promoter activity was measured. C. The mRNA expression levels of IFN-β, OASL, and Mx in DEF cells following poly(I:C) stimulation in the presence or absence of pUS2 were determined by RT-qPCR. D. Expression of pUS2 protein was confirmed by Western blotting analysis.

    Journal: Poultry Science

    Article Title: Duck plague virus US2 promotes p62-mediated autophagic degradation of RIG-I to suppress antiviral signaling

    doi: 10.1016/j.psj.2026.107155

    Figure Lengend Snippet: pUS2 suppresses IFN-β production in DEF cells. A. DEF cells were co-transfected with the IFN-β luciferase reporter plasmid, Renilla luciferase control plasmid, and either pUS2 expression plasmid or empty vector. Cells were stimulated with poly(I:C), and IFN-β promoter activity was measured using a dual-luciferase reporter assay. pUL45, a DPV viral protein, was used as a negative control. B. DEF cells were co-transfected as described above and stimulated with poly(dA:dT). IFN-β promoter activity was measured. C. The mRNA expression levels of IFN-β, OASL, and Mx in DEF cells following poly(I:C) stimulation in the presence or absence of pUS2 were determined by RT-qPCR. D. Expression of pUS2 protein was confirmed by Western blotting analysis.

    Article Snippet: Cells were stimulated with poly(I:C) (MCE, Shanghai, China; Cat# HY-107202) or poly(dA:dT) (Sigma-Aldrich, St. Louis, MO, USA; Cat# P9764) and simultaneously transfected with either the empty vector or the US2 expression plasmid.

    Techniques: Transfection, Luciferase, Plasmid Preparation, Control, Expressing, Activity Assay, Reporter Assay, Negative Control, Quantitative RT-PCR, Western Blot